• Happens during Synthesis (S) phase of cycle
• Human cells contain 46 chromosomes (2 of each kind)
  • Chromosomes get duplicated and become 92 chromatids
• DNA strands are split by proteins, creating forks
  • Single strands are replicated back into double strands
  • Replication happens in both directions
• Strands are split into leading and lagging strands
  • Leading strand is duplicated by simply adding nucleotides
    • Strand is oriented 3’ to 5’, so added from 5’ to 3’ since bases match
  • Lagging strand requires creation of Okazaki fragments, since it goes from 5’ to 3’
    • Strand has to be created from 5’ to 3’, so have to create fragments